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By: Terrence
O'Hanlon
If you are
like me, you strive to reduce the amount of paper in
your life. Now there is a new form of paper you
will want to add to your life if you are involved with
machinery condition monitoring and more specifically,
oil analysis!
Herguth
Laboratories is offering CircOil, a Thin
Layer, Radial, Planar Chromatography analysis tool to
monitor used oil.
William
R. Herguth, CEO of Herguth Laboratories offers the
following explanation of the Science of Thin Layer,
Radial, Planar Chromatography:
That’s
a mouthful of words!
Especially for a simple, quick and very
effective lube oil analysis tool.
There
are many forms of chromatography, but let’s look at
the name in more detail, as it apply to this
technique.
Chromatography
is a technique for separating a sample into its
constituent components and then measuring or
identifying the components in some way. The components
to be separated are distributed between two mutually
immiscible phases. The heart of any chromatograph is
the stationary phase, which is sometimes a solid as in
thin layer chromatography.
The stationary phase is attached to a support,
a solid inert material. The sample is moved across or
through the stationary phase.
The differences in the chemical and physical
properties of the components in the sample are used to
bring about the separation and govern the rate of
movement (called migration) of the individual
components. When
a sample component emerges from the chromatograph, it
is said to have been eluted.
Ideally, components emerge from the system in
the order of their increasing interaction with the
stationary phase.
Separation is obtained when one component is
retarded sufficiently to prevent overlap with the peak
of an adjacent neighbor.
The
term radial refers to the circle formation of sample
constitutes after the sample has eluted on
chromatography substrate, when left on a horizontal
plain. Thus the term; radial planar chromatography.
In
thin-layer chromatography. a stationary phase is
coated on an inert plate of glass, plastic, or metal.
The samples are spotted or placed as streaks on the
plate. Development
of the chromatogram takes place as the mobile phase
percolates through the stationary phase and the spot
locations. The
sample travels across the plate in the mobile phase,
propelled by capillary action.
Separation of components occurs through
adsorption, partition, exclusion, or ion-exchange
processes, or a combination of these.
In
planar chromatography, the position of the resultant
bands or zones, after development, is observed or
detected by appropriate methods.
Because of its convenience and simplicity,
sharpness of separations, high sensitivity, speed of
separation, and ease of recovery of the sample
components, planar chromatography finds many
applications.
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